Time course of TGFbeta induced epithelial mesenchymal transition (EMT) in H358 NSCLC cells.

The capacity of cancer cells to undergo epithelial mesenchymal trans-differentiation has been implicated as a factor driving metastasis, through the acquisition of enhanced migratory/invasive cell programs and the engagement of anti-apoptotic mechanisms promoting drug and radiation resistance. Our aim was to define molecular signaling changes associated with mesenchymal trans-differentiation in two KRas mutant NSCLC models. We focused on central transcription and epigenetic regulators predicted to be important for mesenchymal cell survival. RNA was harvested 0, 1, 2, 4, 6, 8, 18, 24, 72, 168, ~500 and ~4500 hours after TGFbeta addition and subjected to 50bp paired-end Illumina RNAseq analysis. Haley, J.A., Haughney, E., Ullman, E., Bean, J., Haley, J.D.* and Fink, M.Y. (2014) ‘Altered Transcriptional Control Networks with Trans-Differentiation of Isogenic Mutant KRas NSCLC Models’ Front. Oncology, doi/10.3389/fonc.2014.00344.