Sorting FCS files: CRISPR activation library screening with Dendra2-positive cells
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The<b> </b><i>MYC</i> promoter-reporter system was developed with a photoconvertible fluorescent protein, Dendra2, and named “p<i>MYC-</i>promoter-Dendra2.” This <i>MYC</i> promoter-reporter system was designed to harbor a proximal <i>MYC </i>promoter at −3.1 kb. Both the CRISPR activation library and p<i>MYC-</i>promoter-Dendra2 were induced to HEK 293T cells, and Dendra2-positive cells, that are supposed that <i>MYC</i><i> </i>should be upregulated, were collected individually by a cell sorter.<br>
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Morito Kurata创建时间：
2020-04-14
