Comparative analysis of AID-FL and AID-Δex4 mRNA levels.
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An absolute quantification was performed using recombinant DNA-based external standards (Standard Curve Assay Getting Started Guide, Applied Biosystems). Efficiency and linearity of the standard curve (R2) for AID-FL, AID-Δex4, and ACTB were EAID-FL = 1.99, R2AID-FL = 0.999; EAID-Δex4 = 2.03, R2AID-Δex4 = 0.998; EAID-FL = 1.99, R2AID-FL = 0.999; EActin = 2.01, R2Actin = 0.999. Results are expressed as absolute cDNA copy number of target gene per 106 copy numbers of ACTB as HKG. Shown are mean values from three independent experiments each performed in duplicate ± SEM.
创建时间：
2011-10-03
