16S Sequencing of mock community of gut bacterial isolates incubated with a thymidine analogue

Five bacterial isolates originating from human oral and gut samples (Escherichia coli K12 (ATCC 25404), Enterococcus faecalis (SF24397), Prevotella intermedia (ATCC 25611), Bacteroides fragilis (32-6-I 11 MRS AN), and Bifidobacterium longum subsp. infantis (ATCC 15697)) were incubated together in a mock community under anaerobic conditions in vitro with a thymidine analogue (5-ethynyl-2'-deoxyuridine) to fluorescently label newly replicated bacteria. The sequences provided here belong to the mock community after incubation and sorting of labelled and unlabelled cells.