MS2-affinity purification coupled with RNA sequencing (MAPS) reveals SraL sRNA targetome in Salmonella Typhimurium

Recently, we developed an in vivo technology to draw the interacting map of a specific small regulatory RNA (sRNA). We called it MAPS for MS2-affinity purification coupled with RNA sequencing. In this study, we performed MAPS with SraL sRNA (Salmonella Typhimurium SL1344). Overall design: Identification of RNAs co-purified with MS2-SraL in a sraL- strain. SraL (without MS2) was used as control.