The histone acetylation reader ENL is required for oncogenic transcription driven by super-enhancer and represents a synergistic vulnerability for BET inhibition [ChIP-seq]

The histone acetylation reader ENL is identifed as a key factor controlling super-enhancer-driven transcription. Therefore, we constructed a targeted degradation system of endogenous ENL protein and investigated the roles of ENL in regulating oncogenic transcription through epigenetic reprogramming. The ENL gene was taggged by a auxin-inducible degron (AID) in HCT116 colon cancer cells stably expressing E3 ligase AtAFB2. The HCT116 ENL-AID cells was treated with or without 500 um auxin (IAA) for 3 hours and an additional group of IAA treated cells were subjected to PBS washout and subsequent culture in normal medieum for another 48 hours . The resulting cells were harvested for analysis as described below.