Glycolysis inhibition by 2-Deoxy-D-Glucose sustains a distinct cell pluripotency state in human pluripotency stem cells

To investigate the TGFb pathway regulation on glycolysis in hPSCs, we processed glycolysis inhibition (2DG) treamtent in E7 medium (E8-TGFb) for 24h with or without multiple proteins of TGFb pathway in H1. Nodal, Activin A and TGFb were used for treatment with or without 2DG. We performed gene expression profiling analysis using data obtained from RNA-seq of H1 cells following 2DG treatment with or without Nodal, Activin A and TGFb.