Ablation of Histone Demethylase KDM5B in Melanoma Augments Anti-Tumor Immunity through Upregulation of Retroelements [ChIP-Seq]

Purpose: Advanced melanoma patients have poor prognosis. Although immune checkpoint blockade has revolutionized treatment for melanoma patients, majority of patients do not respond. The goal of this research is to evaluate whether epigenetic therapy targeting KDM5B could overcome resistance to immunotherapy. Methods:ChIP-Seq analysis of KDM5B binding on retroelements in mouse melanoma cell line 1445. Results: We identified that KDM5B directly bind to retreoelements to repress their expression. Conclusions: Our work characterized ablation of histone demethylase KDM5B in melanoma augments anti-tumor immunity through Upregulation of retroelements. ChIP-seq analysis of KDM5B in mouse melanoma cell lines; ChIP-qPCR were conducted to verify KDM5B's binding to retroelement loci.