Single-nucleus RNA sequencing-based construction of a hippocampal neuron atlas in mice with epileptic memory impairment

In this study, we utilized snRNA-seq to explore the transcriptional changes in hippocampal neurons of drug-resistant mice with memory disorder in epilepsy, and analyzed the inter-group heterogeneity of 20 subclasses of neurons, focusing on aspects such as cell communication, gene expressions, GO and KEGG enrichment analysis, and module gene set analysis. Our data provides a comprehensive transcriptomic description of the neuronal population affected by drug-resistant epileptic memory disorders. Further studies have shown that specific neuronal subpopulations impact synaptic plasticity through the regulation of relevant target genes by specific transcription factors. Overall design: Comparative gene expression analysis of SnRNA seq data between drug-resistant epileptic memory impairment and normal mouse hippocampal tissue.