Neutrophil activation by IL-4, IL-13 and IFN-g

Interleukin (IL)-4 and IL-13 are related cytokines with well-known roles in type 2 immune response. However, their effects on neutrophils are less defined and existing research has provided somewhat contradictory results We measured IL-4-, IL-13- and IFN-g-stimulated gene expression in highly purified human neutrophils. Human neutrophils purified with Percoll gradient and thereafter sorted from four healthy donors. The purified cells were subsequently stimulated with 100 ng/ml of IL-4 , IL-13 or IFN-γ for 1 hour and the mRNA was extracted and subjected to RNASeq analysis. The gene expression of cytokine-stimulated cells was compared to unstimulated counterparts.