4 micron sections of human thyroid cancer stained with PGP9.5 and counterstained with haematoxylin

Whole slide sections of 4um thickness, from formalin-fixed paraffin embedded blocks of benign and malignant thyroid tissue were labelled with immunohistochemistry for the pan-neuronal marker protein gene-product 9.5 (PGP9.5) using the Ventana Discovery automated slide stainer (Roche Medical Systems, Tuscon, Az), then counterstained with haematoxylin. The primary antibody was anti-rabbit polyclonal PGP9.5 antibody (catalogue number #Ab15503, Abcam, Cambridge, United Kingdom) at 1:600 dilution. Slides were then digitized at 20 X magnification using the Aperio AT2 scanner (Leica Biosystems, Victoria, Australia). The Hunter New England Ethics Committee has approved a subset of the dataset to be publicly available in fully deidentified format for the purpose of validating models for automated detection of nerve tissue. No demographic or clinical details are provided.