Identification and N6-Methyladenosine Modification of the different genes in the peri-infarct cortex of PT mice response to circSCMH1 using MeRIP-seq.

Whole MeRIP-sequencing analysis was performed and analyzed by Lc. Aksomics Co. Ltd. (Shanghai, China). The peri-infarct cortex from sham mice, PT mice with EV-Vector, and PT mice with EV-circSCMH1 was collected in TRIzol. Total RNAs were qualified by agarose gel electrophoresis and quantified using Nanodrop. mRNA was isolated and then fragmented to 100-nucleotide-long fragments. m6A methylated mRNAs were enriched with anti-N6-methyadenosine(m6A) antibody. We use commercial kit for RNA-seq library prepa ration of m6A mRNA and input samples. Completed libraries were qualified and then sequenced on Hiseq 4000 platform. Examination of N6-Methyladenosine Modification by Over-expressed circSCMH1 in the peri-infarct cortex of PT mice by MeRIP-seq.