Nuclear and Cytoplasmic HEK293 Cell 3' end mRNA-seq

Alternative polyadenylation analysis on mRNA from nuclear and cytoplasmic fractions of HEK293 cells RNA was extracted from nuclear and cytoplasmic subcellular fractions of HEK293 cells. The 3’ ends of extracted RNA were generated into libraries using the QuantSeq 3’mRNA-Seq library kit for Ion Torrent (Lexogen). Libraries were loaded onto the Ion Chef System (ThermoFisher) for template preparation and chip loading and the resulting chips were sequenced on the Ion Proton Sequencing System (ThermoFisher). Sequences were aligned using the Ion Torrent Server TMAP aligner to genome build hg19.