RNA-sequencing of WT and Asic3-null leukemia-initiating cells (LICs)

To tease apart the downstream signaling triggered by the Asic3-mediated ion current, RNA-sequencing analyses were conducted with WT and Asic3-null LICs. We found 199 genes were significantly up-regulated, while 587 were down-regulated in Asic3-null LICs. The gene ontology (GO) analysis showed that ASIC3 might mainly be involved in the pattern recognition receptor activity, protein complex binding and calcium ion binding. KEGG further indicated that ASIC3 was required for the glutathione metabolism and hematopoietic cell lineage. Overall design: WT and Asic3-null YFP+Mac-1+c-Kit+ LICs were purified by flow cytometry for the extraction of total RNA and subjected to RNA-sequencing.