Randomization in CyTOF (nonrandomized and maximal data)

Show that randomization can negatively affect multivariate data analysis methods such as dimensionality reduction, clustering and network reconstruction algorithms. Conclusion: Randomization should be used only for visualization purposes, and never in conjunction with high-dimensional analytical tools. Notes: The fcs files refer to the same PBMC data before and after randomization was applied. Please note that the non-randomized data are already normalized and the randomized data are already scaled using arcsinh with co-factor 5.