RNA-seq of 32D clone 3 cells transfected with CXCR2 (pEGFP-N1-CXCR2) or an empty vector (pEGFP-N1) with CXCL1/2 treatment

Purpose: To elucidate a potential role of non-coding RNA in myeloid progenitor cells during the differentiation induced by CXCR2 activation, the 32D clone 3 cells transfected with CXCR2 to simulate GMPs and were examined by RNA sequencing Overall design: To research the function of CXCR2 in the differentiation of myeloid progenitor cells to monocytic myeloid-derived suppressor cells, we used 32D clone 3 cells transfected with CXCR2 to simulate myeloid progenitor cells. Then the cells were incubated with CXCL1 and CXCL2 for 4 h and examined by RNA sequencing. The group that 32D clone 3 cells transfected with an empty vector (pEGFP-N1) was used as the Control group, while the Treated group was transfected with CXCR2 (pEGFP-N1-CXCR2).