Changes of hepatic gene expression in C57BL/6N male mice fed high-fat high-calorie fructose (HFHC) diet for 8 weeks

In murine NAFL/NASH models, high-fat high-calorie fructose (HFHC) diet fed for 16 weeks induces hepatic steatosis, inflammatory cells infilatration and early stage fibrosis. In addition, HFHC diet 16 weeks but not 8 weeks is accompanied with weight gain and insulin resistance. The aim of this study is to determine the gene expression changes in mouse liver by HFHC diet for 8weeks. C57BL/6N male littermates (at age 8 week) were fed ad libitum with Chow or HFHC diet (high-fat high-calorie fructose food plus high fructose drink) for 8 weeks. Liver was collected and freezed in RNAlater RNA stabilizing buffer.