Transcriptomic analysis of engineered cardiac tissue in an oxygen gradient

We report the use of RNA-seq for transcriptional analysis of engineered cardiac tissues cultured in microphysiological systems developed for tight control of oxygen levels, comparing normoxia, hypoxia, and a gradient divided into the normoxic side and hypoxic side. mRNA profiles of engineered cardiac tissues from neonatal rat veintricular myocytes (NRVMs) exposed to normoxia, hypoxia, and a linear oxygen gradient (20-0%)