Microsatellite dataset - foregrounding geodiversity.xlsx

Sampling:Transect sampling was conducted on eastern Marion Island at two sampling areas, namely Nellie Humps (NH) Skua Ridge (SR). The NH site included 24 sampling points separated from each other by a geographic distance ranging from 30 to 120 m, while the SR site comprised 21 sampling points separated by distances ranging from 60 and 190 m. Cryptopygus antarcticus travei specimens were collected by extracting them from sampled moss and/or ferns (approximately 10 cm3) using Berlese-Tullgren funnels. Twenty individuals of C. a. travei from each sampling point were identified and sorted using a compound light microscope, and stored in absolute ethanol (Merck, South Africa).Microsatellite genotyping:Whole genomic DNA was extracted using the DNeasy® Blood and Tissue Kit (Qiagen®, Hilden, Germany), according to the manufacturer's recommendations, with minor modifications that included a longer digestion step (overnight, but not more than 20 hours) and a reduced final elution volume (75 μl instead of 100 μl). Microsatellite loci were amplified in multiplex reactions (seven multiplex sets based on the criteria described by Rastorgueff et al., 2016) using 21 highly variable and species-specific markers (Rastorgueff et al., 2016), and fragment analysis was performed on a MultiGeneTM OptiMax Thermal Cycler (Applied Biosystems). Cleaned PCR amplicons were sized using the ABI Prism® 3500XL Genetic Analyser (Applied Biosystems, Foster City, California, USA). Alleles were analysed, scored, and binned manually using the Geneious v8.1.5 microsatellite plugin 1.4 (Kearse et al., 2012).The dataset added to the repository consists of the scored genotype data for all individuals from both sampling localities.

采样方法：在马里奥尼亚岛的东部地区进行了横断面采样，分别在Nellie Humps（NH）和Skua Ridge（SR）两个采样区域进行。NH站点包含24个采样点，彼此间的地理距离介于30至120米之间，而SR站点则由21个采样点组成，彼此之间的距离介于60至190米之间。通过从样本苔藓和/或蕨类植物（约10立方厘米）中提取Cryptopygus antarcticus travei标本（使用Berlese-Tullgren漏斗），收集了C. a. travei的个体。每个采样点识别并分类了20个C. a. travei个体，使用复合光学显微镜进行，并存储于绝对乙醇（Merck，南非）。微卫星基因分型：采用DNeasy®血液和组织试剂盒（Qiagen®，Hilden，德国）提取全基因组DNA，按照制造商的建议操作，并进行了一些小的修改，包括延长消化步骤（过夜，但不超过20小时）和减少最终洗脱体积（75微升而非100微升）。使用21个高度可变且物种特异性的标记（Rastorgueff等，2016年）在多联反应中扩增微卫星位点（基于Rastorgueff等，2016年所述标准构建了七个多联反应集），并在MultiGeneTM OptiMax Thermal Cycler（Applied Biosystems）上进行片段分析。使用ABI Prism® 3500XL遗传分析器（Applied Biosystems，美国加利福尼亚州福斯特市）对清洗后的PCR扩增子进行定标。等位基因通过Geneious v8.1.5微卫星插件1.4（Kearse等，2012年）手动分析、评分和分箱。添加到存档的数据集包括两个采样地点所有个体的评分基因型数据。