Transcriptomic profiling of thymocytes in CDC42-deficient and wild-type mice using single-cell RNA-seq

We performed single-cell RNA sequencing (scRNA-seq) on thymocytes isolated from wild-type (WT) and CDC42 knockout (CDC42 KO) mice to investigate the role of CDC42 in thymocyte development. This dataset includes raw UMI counts, normalized expression data, and metadata for thousands of thymocytes across different developmental stages. Comparative analysis revealed transcriptional differences between WT and CDC42 KO thymocytes, highlighting CDC42's role in T cell differentiation. Overall design: Thymocytes were isolated from five wild-type (WT) and five CD4-specific CDC42 knockout (CDC42 KO) littermate mice at 6 weeks of age. Single-cell suspensions from each group were pooled to capture a comprehensive transcriptomic profile.