Transcriptome analysis in B16F10 and 4T1 cell lines with decitabine treatment [RNA-seq]

The goal of RNA-seq is to identify the differential expressed genes in the B16F10 and 4T1 cells after Decitabine treatment. Three biological replicates were assigned for each group and in total 12 groups were prepared for RNA-seq libraries with 300 ng mRNA as starting materials using NEXTflex Illumina qRNA-Seq Library Prep Kit (Bioo Scientific). We mapped over 60 million reads per sample to mouse genome (mm10) with RSEM workflow. P-values of differential expression were adjusted for multiple-hypothesis testing using False Discovery Rate (FDR) method. Overall design: Messenger RNA from B16F10 and 4T1 cell lines were extacted and cDNA libraries were generated for deep sequencing, in triplicate, using HiSeq 4000 or NovaSeq S4