Influence of Drosophila gut microbiome structures after topical challenge with different Metarhizium robertsii strains

The 4 DPE (days post eclosion) female flies of Drosophila melanogaster W1118 were collected, anesthetized on ice and topically infected with the spore suspensions of the wild type and transgenic strains of Metarhizium robertsii. The treated insects were kept at a high moisture condition for 12h. After treatment with each fungal strain, five flies each were collected and anesthetized on ice, sterilized and dissected to collect guts, five gut samples were used as one biological repeat. Six repeats were prepared for each fungal strain treatment. Bacterial DNA was extracted and amplified with the 16S V4 primers 515F and 806R. The products were used to generate the amplicon libraries for sequencing with the Illumina Miseq system.