CD177+ neutrophils restrict neuroinflammation following CNS injury

Neutrophils are the most abundant circulating leukocyte population that play critical roles in neuroinflammation after ischemic stroke. CD177, a glycoprotein on neutrophils, is emerging as an important immune regulator which fundamentally affects multiple inflammatory diseases. However, the role and regulatory mechanism of CD177 protein of neutrophils in neuroinflammation remains elusive. Using single-cell RNA sequencing (scRNA-Seq), we characterized CD177high neutrophils as a novel anti-inflammatory subset in response to ischemic stroke. Overall design: Stroke model was induced by middle cerebral artery occlusion (MCAO). scRNA-seq was performed on bone marrow cells from mice after stroke and control conditions.