Meningioma cell lines CH-157MN and IOMM-Lee treated with digitoxin and torin 2

Meningiomas are central nervous system cancers that originate the membranes that surround the brain and spinal cord. To date, there is a lack of effective drug treatment options. This project used a drug combination prediction algorithm to arrive at digitoxin and torin 2 as a potentially effective pairwise combination. RNA-sequencing data was collected from the untreated, digitoxin treated, torin 2-treated, and combination-treated CH-157MN and IOMM-Lee cell lines to experimentally validate the prediction algorithm. Comparative gene expression profiling analysis of RNA-seq data for CH-157MN and IOMM-Lee cells subjected to DMSO, digitoxin (78 nM), torin 2 (2 nM), or digitoxin (78 nM) and torin 2 treatment (2 nM).