Transcriptome analysis of MCF10A-M2 cells upon the depletion of OVOL1 or OVOL2

OVOL1 protein was shown to be downregulated in breast cancer patient samples and was inversely correlated with the epithelial-to-mesenchymal transition (EMT) signature. To better dissect the downstream target genes and elucidate the signaling pathways altered by OVOL1, we deplete OVOL1 by the specific siRNA in MCF10A-M2 cells. To set as a control, OVOL2 was knockdown by the specific siRNA in the same cell line. The total RNA was collected and sent for RNA sequencing analysis. Afterwards, the differentially expressd genes were used for the pathway enrichment analysi and the gene set enrichment analysis (GSEA). Overall design: OVOL1 or OVOL2 is depleted by siRNA in MCF10A-M2 cells. RNA from cells transfected with the non-targeting RNA siNT is set as a control. Three biological replicates are included in each group.