Transient genome-wide adaptations of nascent RNAPII transcription triggered by low temperature

We determined RNA polymerase (RNAPII) transcription at single-nucleotide resolution in Arabidopsis during early stages of exposure to low temperature. Our data inform on the dynamics of RNAPII transcription and detect thousands of often short-lived uncharacterized transcripts. We demonstrate that low temperature triggers transient genome-wide effects on RNAPII activity, in particular the stalling of RNAPII at both gene ends and the distance of read-through transcription. Overall design: Plant native elongating transcripts sequencing (plaNET-Seq) was used to determine the genomic positions of transcriptionally engaged RNAPII in Col-0 seedlings which were exposed to cold (4°C) for 0h, 3h or 12h.