ChIP-Seq H3K4me3 in Jurkat treated with either OICR-9429, PBIT or DMSO

To assess whether BH4Ds are more or less sensitive than sharp H3K4me3 peaks to perturbation of H3K4me3 levels, we performed H3K4me3 ChIP-seq experiments from Jurkat cells treated with DMSO or with either 2-(4-methylphenyl)-1,2-benzisothiazol-3(2H)-one (PBIT), a specific inhibitor of JARID1 family of H3K4me3 demethylases, also known as KDM5, or OICR-9429, an inhibitor of the MLL-WDR5, previously shown to decrease cellular levels of H3K4me3. Overall design: 4 samples in singlicate: 3 ChIP-Seq with spike-in and their input control. The DMSO-treated ChIP sample is used as reference to compare signal variation induced by each inhibitor treatment.