Comprehensive study of immune cells transcriptome and TCR clonotype diversity in mouse lung at different time points following one or two consecutive bacterial infections. Immune cells transcriptome and TCR repertoire following lung bacterial infection

Using single-cell transcriptomic analysis, we comprehensively described the immune composition of C57 Black 6 mouse lung following a bacterial infection at different time points, uninfected (day 0), on-going infection (day 1 and day 3) and infection cured (day 7). Seven days after a first lung bacterial infection, i.e. in cured mice, consequences of a second lung bacterial infection was investigated with the same strategy. Alongside a study of the TCR repertoire of Alpha/Beta T cells, a focus was placed on the transcriptome of Cxcr6 positive T cells following either primary or secondary pneumonia.