CysMAP

Publication: Systematic Cysteine Scanning Identifies a Druggable Pocket in Oncogenic KRAS
OverviewThis dataset contains processed data and metadata associated with the CysMAP study described in the above publication. The contents are derived from intact LC–MS CysMAP screening data deposited at PRIDE under the identifier PXD073201.
These files support reproducibility and downstream analysis of the data presented in the manuscript.
Data origin and processingRaw intact LC–MS data were analyzed using BioPharma Finder software (Thermo Scientific, version 5.1) with default settings.
The BioPharma Finder outputs included here are compatible with the intact-mass utilities available in the CysMAP GitHub repository:
https://github.com/sellerslab/CysMAP/
Folder contentsBioPharma_PDFs.zip
BioPharma Finder PDF reports generated from raw intact LC–MS data. These files contain deconvolution results and charge state assignments and are intended for downstream processing.

CysMAP_ref.xlsx
(Meta)data associated with the intact LC–MS data, including:

A list of all KRAS(G12D) cysteine and serine variants with amino acid sequences, linker annotation, and purification pool allocationPlate barcodes and screening metadataAnnotation of the 47 screening compounds usedAll detected compound binding events (hits) across the intact LC–MS dataset for 182 stable KRAS(G12D) variantsA processed CysMAP reactivity summary for 182 stable KRAS(G12D) variantsCysMAP_protein_expression_and_SEC.pdf
Raw protein expression gel images and size exclusion chromatography (SEC) data.

uncropped_WBs.pdf
Uncropped western blot images corresponding to figures presented in the manuscript.
ContactFor questions regarding these data, please consult the associated manuscript or contact the corresponding authors.