Elucidating the role of UCP1 and FGF21 for metabolic homeostasis in the cold. Mus musculus

Global RNA sequencing analysis of the hypothalamus, BAT, inguinal WAT and muscle of long-term cold exposed WT, FGF21 KO, UCP1 KO and UCP1/FGF21 double KO mice Overall design: The experiment was performed in homozygous, male WT, FGF21 KO, UCP1 KO and UCP1/FGF12 double KO (dKO) mice. All mice were bred, born and weaned at 30°C. At the age of 10 to 12 weeks, mice were transferred to a temperature-controlled (+/- 0.5°C) climate chamber and kept for 12 days at 18°C followed by 3 weeks at 5°C. Afterwards, mice were euthanized after 2-3 hours food withdrawal, and serum and tissue samples were collected.