Genes and pathways regulated by androgens as possible contributors to the male excess observed in autism [RNA-Seq]

We analyzed androgens effects on human neural stem cells (hNSCs) by RNA-sequencing after either DMSO (solvent), DHT 100nM, 10nM, Testosterone 100nM, 10nM, R1881 1nM or retinoic acid 1µM 24 hours treatment in order to find the role of androgen receptor (AR) during brain development. Overall design: Examination of DHT 100nM, 10nM, Testosterone 100nM, 10nM and R1881 1 nM (and retinoic acid 1µM) regulated genes in hNSCs by generating mRNA profiles of DMSO (3 or 4 technical replicates within the 3 biological replicates batches 1, 2 and 3: 10 samples in total) and DHT 100nM (natural metabolite of testosterone, AR agonist, 3 technical replicates within the 3 biological replicate batches 1, 2 and 3: 9 samples in total), DHT 10nM (3 technical replicate of 1 biological serie batch 1), Testosterone 100nM (3 technical replicate of 1 biological serie batch 1), Testosterone 10nM (3 technical replicate of 1 biological serie batch 1), R1881 1nM (3 technical replicate of 1 biological serie batch 1), retinoic acid (3 technical replicate of 1 biological serie batch 1) 24 hours treated hNSCs by deep sequencing using Illumina HiSeq 2500