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Identifying differentially expressed genes in Zmat3 knockout cells

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NIAID Data Ecosystem2026-03-12 收录
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The goal of the study was to identify genes whose expression is Zmat3 depedent using RNA-seq. Overall design: We used CRISPR/Cas9 to knockout Zmat3 in Cas9+ E1A;HrasG12V mouse embryonic fibroblasts (MEFs). After selection for Zmat3 or negative control sgRNAs, cells were cultured for 10 days, and then RNA was collected for RNA-seq, using Illumina TruSeq v2.

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2020-11-07
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