Sequencing of susceptible-turned-resistant strains of Candida albicans, Candida glabrata and Candida tropicalis

Antifungal drug resistance is a global problem. Here, we use whole genome sequence analysis to investigate pairs of isolates from single infection episodes (by definition isolated less than 6 months apart) from individual patients where the initial isolate is susceptible and a subsequent isolate is resistant. The goal was to use whole genome sequence analysis to investigate whether the 2 isolates represented the same strain and to perform comparative genome analysis to elucidate mechanisms of resistance.