THE DNA BINDING LANDSCAPE OF THE MAIZE AUXIN RESPONSE FACTOR FAMILY [DAP-seq]

DAP-seq was used to generate genome-wide DNA:TF interaction maps for fourteen maize ARFs from the evolutionarily conserved class A ‘activator’ and class B ‘repressor’ clades. Among the distinct binding sites that were identified, we observed a high degree of overlap for ARFs of the same class, but found substantial differences in motif sequence, spacing, site preference, and association with auxin induced genes among clade A and clade B ARFs. Genome-wide DNA binding events were determined for fourteen maize ARFs using DAP-seq. Purified genomic DNA from B73 was incubated with purified GST-ARF fusion proteins expressed in Ecoli.