PEMT-mediated phospholipid imbalance promotes age-associated metabolic dysfunction-associated steatotic liver disease progression

Metabolically-dysfunction-associated steatotic liver disease [MASLD; previously known as nonalcoholic fatty liver disease (NAFLD)] is a prevalent chronic liver disorder strongly associated with aging, yet the mechanisms underlying age-related hepatic lipid dysregulation remain incompletely defined. This study investigates how aging alters hepatic phospholipid metabolism and the contribution of phosphatidylethanolamine N-methyltransferase (PEMT) to MASLD progression. Here, lipid accumulation was characterized in the livers of 2-, 6-, 12-, 18-, and 24-month-old mice, revealing age-dependent increases in hepatic triglyceride (TG), total cholesterol (TC), and lipid droplet formation. Lipidomic analysis revealed a marked imbalance in phospholipid composition, characterized by increased phosphatidylcholine (PC) and decreased phosphatidylethanolamine (PE), resulting in a 2.5-fold increase in the PC/PE ratio in 24-month-old mice compared to 6-month-old controls. Mechanistically, PEMT, a key enz..., , # PEMT-mediated phospholipid imbalance promotes age-associated metabolic dysfunction-associated steatotic liver disease progression

Dataset DOI: [10.5061/dryad.7d7wm386j](https://doi.org/10.5061/dryad.7d7wm386j)

## Description of the data and file structure

This dataset contains quantitative lipidomics profiling data derived from liver tissues of C57BL/6J mice. The experimental objective was to investigate age-associated alterations in the hepatic lipidome and to elucidate the role of PEMT-mediated phospholipid imbalance in the progression of metabolic dysfunction-associated steatotic liver disease (MASLD). Liver samples were collected from two specific age groups: young mice (6 months old, denoted as 6M) and aged mice (24 months old, denoted as 24M). Total lipids were extracted from the liver tissues and analyzed using liquid chromatography-mass spectrometry (LC-MS). The provided data includes the identification and quantification (in nmol/g) of various lipid species, highlighting s...,