Generation of Peptide Libraries: Applying Various In silico Enzymatic Digestion Protocols on the Composite Protein Database (nr).

Peptide Diversity Generation through In silico Enzymatic Digestion of the Composite Protein Database (nr) from Cephalopod's Posterior Salivary Glands resulted in 13 distinct peptide libraries. These libraries were created using various digestion protocols, including just one enzyme (Trypsin, Chymotrypsin, Proteinase-K, AspN, and GluC), sequential mode with two enzymes (Tryp-Chym, Tryp-ProtK, Tryp-AspN, and Tryp-GluC), and concurrent mode with two enzymes (Tryp-Chym, Tryp-ProtK, Tryp-AspN, and Tryp-GluC).