Cellular and Molecular Profiling of Collagenous Gastritis

Collagenous gastritis (CG) is a rare disease characterized by thick bands of subepithelial collagen within the stomach and presents with a variety of symptoms ranging from anemia to vomiting. Gastric biopsies were collected from six CG patients, including one patient before and after glucocorticoid steroid treatment, and several matched controls. Single-cell RNA- and T-cell receptor (TCR)-sequencing was performed on gastric biopsies. Overall design: Control or CG patient biopsies were processed fresh and unsorted or frozen and CD45+ live flow-sorted. Single cell suspensions where processed in the 10x Genomics pipeline for scRNA- and scTCR-sequencing.