Cells-In-Wells

Cells-In-Wells (CIW) contains images, videos, binary masks, bounding boxes, morphology labels, cell tracking data, and other metrics of red blood cells (RBCs) in microfluidic wells undergoing washing in human serum albumin, saline, or autologous storage medium. RBC units are from six different donors, which have been leukoreduced and stored in a blood bank refrigerator (2°C to 6°C) for 4, 5, or 6 weeks: donors 1 & 2 are four weeks old, donors 3 & 5 are five weeks old, and donors 4 & 6 are six weeks old. Images were acquired at 40 X magnification and at a rate of 1 frame per second. We derived all other data by applying an in-house image analysis pipeline. This dataset shows the impact that washing has on RBC shape recovery, and we recommend its employment for the training & testing of machine learning models. Also, one could apply their own analysis to gain new biologically relevant knowledge of RBC washing.