Gene expression profiling of triple-negative breast cancer cells by Nitidine Chloride(NCD)

To obtain the gene expression profiles of triple-negative breast cancer MDA-MB-231 cells perturbed by NCD, we treated MDA-MB-231 with either DMSO or 5 µM NCD for 24 hours. By comparing the expression profiles of DMSO-treated cells with those treated with Nitidine Chloride, we can identify the genes affected by Nitidine Chloride. Overall design: MDA-MB-231 cells were treated with 0.1% DMSO or 5 µM NCD for 24 hours followed by RNA isolation