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Supplementary Material: Defining Chaperone-Usher Fimbriae Repertoire in Serratia marcescens

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Figshare2021-01-06 更新2026-04-08 收录
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Supplementary Material<br><br>Supplementary File 1. Fimbrial Usher Protein (FUP) consensus sequences for α, β, γ1, γ2, γ3, γ4, κ, and π families were built with cons tool (http://www.bioinformatics.nl/cgi-bin/emboss/cons) and used as query in a tBLASTn against S. marcescens genomes.<br><br>Supplementary File 2. Genomic coordinates of S. marcescens FUP-coding genes. Each row corresponds to a S. marcescens sequenced strain. Each CU operon family and given name is indicated on top. Nucleotide start of each FUP-coding gene in the associated genome is indicated in individual cells.<br><br>Supplementary File 3. CLUSTALX v2.0 alignment of the 421 FUP of S. marcescens along with 189 FUP from different Gram-negative bacteria. The S. marcescens isolate name along with the here assigned CU fimbriae nomenclature associated is indicated. GenBank accession numbers of non-S. marcescens FUPs along with their previously assigned family are also indicated.<br><br>Supplementary File 4. Growth curves at 37°C of S. marcescens SmUNAM836 (A) or Db10 (B) strains, carrying pSEVA246 plasmid. Data represent the mean ± SD from two independent experiments.
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