Genetic dissection of drought tolerance in chickpea (Cicer arietinum L.)

Screening of 2,717 SSR markers on the parental lines resulted in identification of 321 and 230 polymorphic markers on ICCRIL03 and ICCRIL04, respectively (Lichtenzveig et al. 2005; Varshney et al. 2009; Nayak et al. 2010; Gujaria et al. 2011; Thudi et al. 2011; ESM Table S2). The genotyping data were generated for the polymorphic markers on respective mapping populations. 241 marker loci including 214 SSRs, 6 GMMs and 21 DArT loci were placed on to genetic map for ICCRIL03 and 168 marker loci (151 SSRs, 10 GMMs and 7 DArT loci) in the case of ICCRIL04. In total, 62 (26.95 %) markers in the case of ICCRIL04 and 80 (24.92 %) markers in the case of ICCRIL03 remained unmapped. Varying levels of marker densities were recorded for different linkage groups (LGs) in both the maps and the average inter-marker distances were 2.71 and 3.27 cM in the case of ICCRIL03 and ICCRIL04, respectively.