LFY is pioneer transcription factor [ChIP-seq]

This study identified LEAFY (LFY) as a pioneer transcription factor. We made use of 35S:LFY-GR, an inducible version of the LFY protein fused to the rat glucocorticoid hormone binding domain. In root explants, steroid activated LFY-GR triggers synchronous and abundant flower induction (PMID: 15225291). We combined LFY ChIP-seq, MNase-seq before and after LFY binding and time-course RNA-seq in 35S:LFY-GR root explants to characterize the role of LFY as a pioneer transcription factor. Identification of genomic regions bound by LFY in root explants identified using 35SLFY-GR in L.er ecotype of Arabidopsis thaliana. ChIP seq was performed using anti LFY antibodies 24 hours after dexamethasone or mock treatment. Three biological replicates were sequenced for each condition, as were three inputs.