Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [Pax5-ChIP-Seq-Tet-On]

EBF1 is essential for B cell specification and commitment. To explore the dynamics of EBF1 initiated B cell programming, we performed EBF1 ChIP-seq, ATAC-seq, bisulfite-seq, RNA-seq and several histone ChIP-seq analyses at different stages of the transition from Ebf1-/- pre-pro-B to pro-B triggered by EBF1 restoration. We also performed Pax5 ChIP-seq in Ebf1-/- pre-pro-B cell and EBF1-restored pro-B cell to study the pioneering function of EBF1 that allows other transcription factors to access certain chromatin sites. Pax5 ChIP-Seq analysis in Ebf1-deficient pre-pro-B cell and EBF1-restored pro-B cell.