Pseudomonas fluorescens strain:51486_Pf0-1_Pf0-2x_glnA-G1249C | isolate:Pf0-2x Genome sequencing and assembly. Pseudomonas fluorescens strain:51486_Pf0-1_Pf0-2x_glnA-G1249C | isolate:Pf0-2x

We placed immotile variants of the soil bacterium Pseudomonas fluorescens Pf0-1 (Pf0-1(delta)fleQ; Pf0-2x) under selection for recovered flagella-mediated motility using soft agar as a directional selection medium. We observed that typically a single de novo mutation would arise within 72-144 hours and restore the motility phenotype. We sent a subset of evolved lines from this experiment for whole-genome sequencing to identify the phenotype-causing mutation and screen for secondary, background and hitchhiking mutations. One mutant line was identified to harbour a single nucleotide polymorphism within the glnA locus (glnA G1249C), which is a member of the nitrogen regulatory pathway associated with recovery of the flagella-mediated motility phenotype.