Draft genome dataset of the Antarctic pearlwort Colobanthus quitensis
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https://figshare.com/articles/dataset/Colobanthus_genome_fasta_Functional_annotation/25603194
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This dataset includes the genome reference fastfile (including proteins & transcripts) and annotation gff file generated as part of a study titled "Exploring the cellular and molecular mechanism underlying recovery from freeze-thaw injury in C. quitensis (Kunth) Bartl.: mechanistic insights via transcriptome profiling". The genome reference fastfile contains the assembled genome sequences generated using 10x Genomics Chromium technology according to the manufacturer's instructions, followed by process of Illumina library construction, as detailed in the Chromium Genome Reagent Kits Version 2 User Guide (PN-120258); subsequently, the DNA was sequenced on a NOvaSeq with a 2 x 250 bp read metric, and ~1.6 billion paired-end (PE) reads were generated, after which the de novo genome assembly was performed using Supernova assembler (PRID:SCR 016756) v2.1.1 (10x Genomics, San Francisco, CA, USA) with default parameters. The annotation gff file provides genes annotated based on protein sequence via BLASTP diamond wherein the filtering option with e-value cutoff (1e-5) was applied for mapping the best hit proteins.
创建时间:
2024-04-15



