Diverse, distinct, and densely packed DNA nanostar droplets

The liquid-liquid phase separation of biomolecules is an important process for intracellular organization. Biomolecular sequence combinatorics leads to a large variety of proteins and nucleic acids which can interact to form a diversity of dense liquid ('condensate') phases. The relationship between sequence design and the diversity of the resultant phases is therefore of interest. Here, we explore this question using the DNA nanostar system, which permits creation of multi-phase condensate droplets through sequence engineering of the sticky end bonds that drive particle-particle attraction. We explore the theoretical limits of nanostar phase diversity, then experimentally demonstrate the ability to create 9 distinct, non-adhering nanostar phases that do not share components. We further study how thermal processing affects the morphology and dynamics of such a highly diverse condensate system. We particularly show that a rapid temperature quench leads to the formation of a densely packe..., , # Data from: Diverse, distinct, and densely packed DNA nanostar droplets

Dataset DOI: [10.5061/dryad.7d7wm387m](10.5061/dryad.7d7wm387m)

## Description of the data and file structure

This document describes the data repository associated with the paper Diverse, Distinct, and Densely Packed DNA Nanostar Droplets by Aria S. Chaderjian, Sam Wilken, and Omar A. Saleh.

### Files and variables

This repository consists of a .zip archive containing 51 data files, as well as a Mathematica notebook and its corresponding PDF.

The .zip ( 2025-01-29-DiverseNanostars-DataArchive.zip) contains the following files:

-This ReadMe file

-Three files containing all 4-, 6-, and 8-base DNA palindromes with Delta G values calculated from the 2004 SantaLucia parameters (see the Mathematica notebook GraphStructureOfOrthogonalPalindromes-ForRelease.nb). These files underlie Fig. 1D of the main text.

     Filenames:

* AllFourBasePalindromesWithDG.csv
* AllSixBasePalindromesWithDG.csv
* AllEightBasePalin...,