Supplementary Figure 1. UL12.5 is expressed early during Phase I of reactivation and co-localize with mitochondria.

S1A. Representative immunofluorescence images (TIFs) of UL12/UL12.5 (BWp12) co-localization with mitochondria (TOM20) in SCGs following reactivation as early as 8 hours post stimuli. Primary neurons latently infected with either KOS-SPA or KOS-UL98 were reactivated with LY294002 (20 µM) and fixed 8- and 20- hours post-stimuli.S1B. Quantification of UL12.5 expressing neurons as a percentage of neurons per 10 counted cells at 8- and 20- hours after reactivation (50 cells per condition).S1C. Representative immunofluorescence images (TIFs) of UL12/UL12.5 (BWp12) and mitochondria (TOM20) co-localization following transduction with lentivirus expressing either GFP or UL12.5. Primary neurons were fixed at 72- hours post-transduction.S1D. Validation of BWp12 anti-sera specificity in primary murine neurons. Immunofluorescence images (TIFs) of UL12/UL12.5 (BWp12) and FLAG (UL12.5) co-localization following transduction with lentivirus expressing either GFP or UL12.5. Primary neurons were fixed at 72- hours post-transduction.S1E. Representative image showing intersection of UL12/12.5 (BWp12) and FLAG (UL12.5) overlap. Dataset for histogram showing the overlap of signal intensity distribution across intersection for anti-UL12/12.5 - BWp12 (Green) and anti-FLAG antibody (Red).