Gene Expression Analysis of Laser Captired Purkinje Cells from the Post-Mortem Essential Tremor Cerebellum

RNA-seq evaluation of enriched Purkinje cells from the post-mortem human cerebellum. Purkinje cells were removed via laser capture microdissection and pooled for RNA-extraction and sequencing. 24 ET patients and 16 controls healthy age matched were compared. Overall design: The Purkinje cell has shown extensive morphological changes in the ET cerebellum, but are less abundant than other cell types (Ex: Granule cells) by a factor of about 1:600. We sought to sequence enriched Purkinje cells from patients diagnosed with Essential Tremor and patients who are neurologicall clear.