Data from: Open-top Bessel beam two-photon light sheet microscopy for three-dimensional pathology

Nondestructive pathology based on three-dimensional (3D) optical
microscopy holds promise as a complement to traditional destructive
hematoxylin and eosin (H&E) stained slide-based pathology by
providing cellular information in high throughput manner. However,
conventional techniques provided superficial information only due to
shallow imaging depths. Herein, we developed open-top two-photon light
sheet microscopy (OT-TP-LSM) for intraoperative 3D pathology. An extended
depth of field two-photon excitation light sheet was generated by scanning
a nondiffractive Bessel beam, and selective planar imaging was conducted
with cameras at 400 frames/s max during the lateral translation of tissue
specimens. Intrinsic second harmonic generation was collected for
additional extracellular matrix (ECM) visualization. OT-TP-LSM was tested
in various human cancer specimens including skin, pancreas, and prostate.
High imaging depths were achieved owing to long excitation wavelengths and
long wavelength fluorophores. 3D visualization of both cells and ECM
enhanced the ability of cancer detection. Furthermore, an unsupervised
deep learning network was employed for the style transfer of OT-TP-LSM
images to virtual H&E images. The virtual H&E images
exhibited comparable histological characteristics to real ones. OT-TP-LSM
may have the potential for histopathological examination in surgical and
biopsy applications by rapidly providing 3D information.