Comparison of Pairwise Venous and Fingertip Plasma Using Quantitative Proteomics Based on Data-Independent Acquisition

Blood
contains a great deal of health-related information and can
be used to monitor human health status. Clinically, venous or fingertip
blood is usually used for blood tests. However, the clinical application
settings of the two sources of blood are unclear. In this study, the
proteomes of pairwise venous plasma (VP) and fingertip plasma (FP)
were analyzed, and the levels of 3797 proteins were compared between
VP and FP. The Spearman’s correlation coefficient for the relationship
between protein levels of VP and FP ranges from 0.64 to 0.78 (p < 0.0001). The common pathways of VP and FP are related
to cell–cell adhesion, protein stabilization, innate immune
response, and complement activation, the classical pathway. The VP-overrepresented
pathway is related to actin filament organization, while the FP-overrepresented
pathway is related to the hydrogen peroxide catabolic process. ADAMTSL4,
ADIPOQ, HIBADH, and XPO5 both in VP and FP are potential gender-related
proteins. Notably, the VP proteome has a higher interpretation on
age than the FP proteome, and CD14 is a potential age-related protein
in VP but not in FP. Our study mapped the different proteomes between
VP and FP, which can provide value for the standardization of clinical
blood tests.